Apparatus

Topics in Apparatus: Pulsed and Reverse Field | Micro Electrophoresis | Capillary Electrophoresis | Continuous Flow | Automated Analyzers DNA | all topics

 

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Protein Electrophoresis (12105)

Easy to assemble and able to run up to four SDS-PAGE mini-gels simultaneously, the Mini-PROTEAN&reg Tetra electrophoresis system offers leak-free hand casting and can run either hand-cast or precast gels.

Fractionate Proteins, Peptides By Isoelectric Point

The 3100 OFFGEL Fractionator fractionates intact proteins or peptides by isoelectric point (pI) in liquid phase. Sample complexity is reduced, while the sensitivity of LC/MS analysis is greatly increased. Fractionation is pI-based, with 0.1 – 0.

Electrophoresis Mini-gel System (11121)

The Mini-PROTEAN Tetra Cell 1-D electrophoresis mini-gel system is offered for both hand-cast and pre-cast gel users and features a clamping frame. Using two running modules, the system accommodates from one to four hand-cast or Ready Gel pre-cast gels.

Electrophoresis Mini-gel System

The Mini-PROTEAN Tetra Cell 1-D electrophoresis mini-gel system is offered for both hand-cast and pre-cast gel users and features a clamping frame. Using two running modules, the system accommodates from one to four hand-cast or Ready Gel pre-cast gels.

High Throughput RNA Quality Control

High Throughput RNA Quality Control

Determining the integrity of RNA is an essential step before gene expression analysis, because RNA quality can have a tremendous effect on downstream microarray analysis, data interpretation of gene expression profiling, and disease diagnosis. In addition to microarray analysis, real time PCR amplification analysis, reverse transcriptional experiments, and northern blot analysis rely on the high quality of the RNA samples.

Electrophoresis Products

Electrophoresis Products

Injection molded electrophoresis product includes horizontal and vertical gel electrophoresis

Mini Gel Electrophoresis

Mini Gel Electrophoresis

The Gel XL Ultra V-2 mini gel electrophoresis system includes an intelligent power supply, migration tank and gel casting sets. The unit and direct-connect power supply take up less than ½ sq/ft of bench space. The migration tank plugs directly into the power supply.

Mutation Detection Kits

Mutation Detection Kits

SURVEYOR Mutation Detection Kits, for universal primer capillary electrophoresis, contain two key components: SURVEYOR Nuclease, a mismatch-specific endonuclease derived from celery, and fluorescent universal FKS primer, which can be used to label any PCR product.

Pre-coat Reagents For Capillary Electrophoresis

Pre-coat Reagents For Capillary Electrophoresis

CE has historically struggled with limitations caused by lack of control over electroosmotic force and flow (EOF), a natural occurrence in CE separations. UltraTrol products address difficult separation hurdles to overcome technical limitations in biochemical separation and identification.

Electrokinetic Migration Technology

Electrokinetic Migration Technology

The EMP-15 D/A routinely delivers electrokinetic migrations of molecular species that take just seconds and only require movements of a few centimeters or less.

Research Exchange

Publishing Data That Conform to the MIQE Guidelines

Jan 22

Minimum information for publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines help researchers design qPCR experiments.

Calibration Free Analysis to Measure the Concentration of Active Proteins

Nov 23 2009

An SPR-based method, Calibration Free Concentration Analysis can be used to accurately determine the concentration of active protein in a sample, relating to the specific binding activity of the protein, and without the need for a standard.

Advances in EMCCD Technology: Making Imaging Less Arbitrary

Advances in EMCCD Technology: Making Imaging Less Arbitrary

Nov 16 2009

Recent advances in EMCCD technology have solved the problem of non-standardized measurement units by using the photoelectron to standardize imaging experiments.

10 Tips for Successful Sample Concentration and Buffer Exchange

10 Tips for Successful Sample Concentration and Buffer Exchange

Nov 6 2009

Centrifugal devices with ultrafiltration (UF) membrane can solve common problems researchers face when working with proteins.

Advantages of Monolithic Laser Combiner Technology in Confocal Microscopy Systems

Jan 6

Fluorescence microscopy techniques require a reliable light source at the desired wavelength or wavelengths, with minimal downtime for maintenance and alignment. Lasers are a popular light source, although the alignment and upkeep of laser combiners is a time-consuming prospect for many users.

Size-Exclusion Chromatography for Purification of Biomolecules

Dec 2 2009

Size-exclusion chromatography (SEC) is a popular method to separate biomolecules based on their size. Primarily, it is applied to the separation of biopolymers such as proteins and nucleic acids, i.e. water-soluble polymers.

Improving Separation During Electrophoresis

Dec 2 2009

SeparateIT gels represent a novel gel matrix for DNA electrophoresis. Gel polymers are arranged in a conceptually different way, in accordance with a new theoretical model of gel electrophoresis.

Improving Quality of ELISA

Dec 2 2009

Using ready-to-use ELISA kits from manufacturers is easy and convenient. Sometimes however, home-made ELISA is required because there is no kit available with the right antibodies or the characteristics of the available kits such as their limits of detection are not appropriate.

Using the Tecan Genesis Workstation to Automate a Cytometric Bead Array (CBA) Immunoassay

10 hours ago

The poster describe the process involved in automating a Cytometric Bead Array (CBA) immunoassay developed to measure relative concentrations of serum antibodies against Tetanus (TT), Sperm Whale Myoglobin (SWM) and Keyhole Limpet Hemocyanin (KLH) in KLH-immunized volunteers.

Ensuring Quality in Assays Performed with Automated Liquid Handlers

Feb 2

The focus of this presentation is to highlight the need of ensuring quality in important assays performed with automated liquid handlers. Nearly all assays performed within a laboratory are volume-dependent. In turn, all concentrations of biological and chemical components in these assays, as well as the associated dilution protocols, are volume-dependent. Because analyte concentration is volume-dependent, an assay’s results might be falsely interpreted if liquid handler variability and inaccuracies are unknown or if the system(s) go unchecked for a long period.

Inkjet System for Protein Crystallography

Feb 1

X-ray crystallography is used routinely by scientists to obtain the three dimensional structure of a biological molecule of interest.Such information can be used to determine how a pharmaceutical interacts with a protein target and what changes might improve functionality. However, the crystallization of macromolecules still remains a serious hindrance in structural determination despite impressive advances in screening methods and technologies.

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Nov 9 2009

Action selection is modulated by external stimuli either directly or via memory retrieval. In a constantly changing environment, animals have evolved attention-like processes to effectively filter the incoming sensory stream. These attention-like processes, in turn, are modulated by memory. The neurobiological nature of how attention, action selection and memory are inter-connected is unknown. We describe here new phenotypes of the memory mutant radish in the fruit fly Drosophila.

Relationship between multiple sources of perceived social support and psychological and academic adjustment in early adolescence: comparisons across gender.

Mar 8

The current study investigated gender differences in the relationship between sources of perceived support (parent, teacher, classmate, friend, school) and psychological and academic adjustment in a sample of 636 (49% male) middle school students. Longitudinal data were...

Involvement of ceramide in ischemic tolerance induced by preconditioning with sublethal oxygen-glucose deprivation in primary cultured cortical neurons of rats.

Feb 25

The complex molecular cascades of ischemic tolerance in brain cells remain unclear. Recently, sphingolipid-related metabolite ceramide has been implicated as a second messenger in many biological functions, including neuronal survival and death. The present study, therefore,...

Social intelligence and academic achievement as predictors of adolescent popularity.

Feb 24

This study compared the effects of social intelligence and cognitive intelligence, as measured by academic achievement, on adolescent popularity in two school contexts. A distinction was made between sociometric popularity, a measure of acceptance, and perceived popularity, a...

Prokariotic Cell Collection in Denmark

Nov 6 2009

I would like to know about a prokariotic cell collection in Denmark. Is there a cell bank in this country? I need a Lactobacillus strain for a fermentation assay and this information about the bank is very helpful for me.

Request for Entries

Oct 16 2009

Ask the Experts is your chance to get the answers to questions on applications, materials, methods, processes, and technologies. Email you question to bst_web@advantagemedia.com, and the editors of Bioscience Technology will find an appropriate expert to answer it. Watch this space in the future to see the questions your colleagues are posting.          

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Video:

Neuroscience Diseases of The Brain and How The Mind Emerges

Neuroscience Diseases of The Brain and How The Mind Emerges

Nov 8 2009

Dennis Choi, director of Emory Universitys Neuroscience Center, is renowned for his groundbreaking research on brain and spinal cord injury.

Podcasts:

Allen Institute for Brain Research

Allen Institute for Brain Research

Oct 14 2009

Discussed in this interview are both the mouse brain project and the human cortex project with an emphasis on the importance of these projects to neuroscience research.